CD31 anticorps

N° du produit ABIN6252198

Cet anticorps Souris Monoclonal détecte spécifiquement CD31 dans FACS, WB, IHC et IF. Il présente une réactivité envers Humain.

Aperçu rapide pour CD31 anticorps (ABIN6252198)

Antigène: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD31 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: PCM25-1

Détail du produit anti-CD31 anticorps

Purification: Protein G affinity chromatography

Immunogène: Recombinant human protein was used as the immunogen for this CD31 antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Western blot: 1-2 μg/mL

Flow Cytometry: 0.5-1 μg/10e6 cells

IF: 1-2 μg/mL

IHC (FFPE): 1-2 μg/mL for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Titering of the CD31 antibody may be required for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 1  mM EDTA,  pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur CD31

Antigène: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Autre désignation: CD31

Sujet: CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [RefSeq]

ID gène: 5175

UniProt: P16284

Pathways: Regulation of Actin Filament Polymerization